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About Transplantation Immunology

 

Testing items

Specimen types

Clinical significance of laboratory tests


Lymphocyte crossmatch

 

Recipient: serum

Donor: ACD Whole blood

This method detects the presence of antibodies in the recipient's serum that correspond to the donated tissue antigen HLA. The fewer dead lymphocytes, the better the tissue compatibility. If the cell death rate exceeds 15%, hyperacute rejection will occur after transplantation. This method has become a routine examination for most clinical transplants before transplantation.

 

Lymphocyte surface marker for-infectious disease

(CD3﹑4﹑8﹑19)

 

EDTA Whole blood

CD4/CD8 monitoring commonly used for AIDS patients.

Lymphocyte surface marker for others /transplantation

 (CD3﹑4﹑8﹑19﹑16+56)

 

EDTA Whole blood

It mainly analyzes the condition of patients after transplantation and evaluates the immune status of patients with autoimmune diseases.

Panel reactive antibody screening﹐PRA

 (flow)

serum

This test detects the presence of group-reactive antibodies against human leukocyte antigens in the serum of organ transplant recipients. It is used to monitor postoperative rejection reactions in organ transplant recipients.

HLA-B27

(flow)

 

EDTA Whole blood

There is a high correlation between the HLA-B27 antigen and ankylosing spondylitis. More than 90% of ankylosing spondylitis patients test positive for the HLA-B27 antigen.

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