TEL: +886-2-2871-2121 #4206

E-mail: sjchou3@vghtpe.gov.tw

2010 ~ 2016  國立陽明大學藥理所博士

2016 ~ 2018  台北榮總醫研部博士後研究員

2018 ~ 2019  加州大學洛杉磯分校訪問學者

2019 ~ present  台北榮總醫研部契約高級助理研究員

My research interests have been devoted to exploring nanomaterials-enabled technologies for biomedical applications, for examples treatment of genetic disease and development of vaccines. More specifically, my research has been working to apply reprogramming technique to generate patient-specific hiPSC as an in vitro model for investigating the pathophysiologic mechanism of inherited disease, e.g., MERRF syndrome, Fabry disease, cystic fibrosis. In the meantime, I also established an extensive collaboration network with UCLA for exploiting the use of supramolecular nanoparticle (SMNP) vectors for co-delivery of CRISPR/Cas9-based gene editing system (including Cas9 protein, single-guide RNA, and donor DNA plasmid) to precisely modify the nucleotide sequence for treating genetic diseases, e.g., beta hemoglobinopathies and X-linked juvenile retinoschisis.

1. 藉由奈米粒子運輸CRISPR/Cas9，在幹細胞誘導的類器官中進行基因治療。
2. 將誘導多能幹細胞分化成肺類器官，並進行遺傳性疾病及傳染性疾病機制探討。
3. 建立細胞與小鼠黃斑部病變模型，並進行藥物篩選治療。

國科會：

110-2314-B-075 -066 -

110-2320-B-075 -010 -

109-2320-B-075 -009 -

榮台聯大合作計畫：

視網膜色素上皮細胞移植至RCS大鼠後電生理反應和動物行為測試

院內計畫：

V110B-020

V111B-015

嚴慶齡基金會：

CI-110-25

2020 台北榮總創新改良獎

2020 國家新創獎

2021 台北榮總學術論文獎

2021 科技部未來科技獎

* Equal contribution

26.  Lee MS, Chien Y, Teng PC, Huang XY, Lin YY, Lin TY, Chou SJ, Chien CS, Hsiao YJ, Yang YP, Hsu W, Chiou SH. Superrepellent Doubly Reentrant Geometry Promotes Antibiofouling and Prevention of Coronavirus Contamination. Adv Mater Technol. 2022 Aug 5;2200387.

25.  Afshar Y, Dong J, Zhao P, Li L, Wang S, Zhang RY, Zhang C, Yin O, Han CS, Einerson BD, Gonzalez TL, Zhang H, Zhou A, Yang Z, Chou SJ, Sun N, Cheng J, Zhu H, Wang J, Zhang TX, Lee YT, Wang JJ, Teng PC, Yang P, Qi D, Zhao M, Sim MS, Zhe R, Goldstein JD, Williams J 3rd, Wang X, Zhang Q, Platt LD, Zou C, Pisarska MD, Tseng HR, Zhu Y. Circulating trophoblast cell clusters for early detection of placenta accreta spectrum disorders. Nat Commun. 2021 Aug 3;12(1):4408.

24.  Sun N, Yang Y, Miao H, Redublo P, Liu H, Liu W, Huang YW, Teng PC, Zhang C, Zhang RY, Smalley M, Yang P, Chou SJ, Huai K, Zhang Z, Lee YT, Wang JJ, Wang J, Liang IY, Zhang TX, Zhang D, Liang L, Weiss PS, Posadas EM, Donahue T, Hecht JR, Allen-Auerbach MS, Bergsland EK, Hope TA, Pei R, Zhu Y, Tseng HR, Heaney AP. Discovery and characterization of circulating tumor cell clusters in neuroendocrine tumor patients using nanosubstrate-embedded microchips. Biosens Bioelectron. 2022 Mar 1;199:113854.

23.  Khor W, Hwang TC, Wang CC, Yarmishyn AA, Yeh JT, Chiou SH, Chou SJ. Generation of human induced pluripotent stem cells from cystic fibrosis patient carrying nonsense mutation (p.S308X) in CFTR gene. Stem Cell Res. 2022 Jan 20;60:102683.

22.  Tsai HY, Lai HIAM, Chen ZY, Lin TC, Khor W, Kuo WC, Syu JP, Tsai PH, Yang YP, Chien Y, Chen SJ, Hwang DK, Chiou SH, Chou SJ. Inhibition of DUSP6 Activates Autophagy and Rescues the Retinal Pigment Epithelium in Sodium Iodate-Induced Retinal Degeneration Models In Vivo and In Vitro. Biomedicines. 2022 Jan 12;10(1):159.

21.  Kuo MH, Chen PY, Yang YP, Zheng MY, Miao CC, Wen KC, Chang KM, Chou SJ, Wang ML, Chiou SH, Chou YT. Cytokine and epigenetic regulation of programmed death-ligand 1 in stem cell differentiation and cancer cell plasticity. Stem Cells. 2021 Oct;39(10):1298-1309.

20.  Ban Q, Yang P, Chou SJ, Qiao L, Xia H, Xue J, Wang F, Xu X, Sun N, Zhang RY, Zhang C, Lee A, Liu W, Lin TY, Ko YL, Antovski P, Zhang X, Chiou SH, Lee CF, Hui W, Liu D, Jonas SJ, Weiss PS, Tseng HR. Supramolecular Nanosubstrate-Mediated Delivery for CRISPR/Cas9 Gene Disruption and Deletion. Small. 2021 Jun 8;e2100546.

19. Ahmad Mulyadi Lai HI, Chou SJ, Chien Y, Tsai PH, Chien CS, Hsu CC, Jheng YC, Wang ML, Chiou SH, Chou YB, Hwang DK, Lin TC, Chen SJ, Yang YP. Expression of Endogenous Angiotensin-Converting Enzyme 2 in Human Induced Pluripotent Stem Cell-Derived Retinal Organoids. Int J Mol Sci. 2021 Jan 28;22(3):1320.

18. Yarmishyn AA, Yang YP, Lu KH, Chen YC, Chien Y, Chou SJ, Tsai PH, Ma HI, Chien CS, Chen MT, Wang ML. Musashi-1 promotes cancer stem cell properties of glioblastoma cells via upregulation of YTHDF1. Cancer Cell Int. 2020 Dec 14;20(1):597. doi: 10.1186/s12935-020-01696-9.

17. Yang P*, Chou SJ*, Li J, Hui W, Liu W, Sun N, Zhang RY, Zhu Y, Tsai ML, Lai HI, Smalley M, Zhang X, Chen J, Romero Z, Liu D, Ke Z, Zou C, Lee CF, Jonas SJ, Ban Q, Weiss PS, Kohn DB, Chen K, Chiou SH, Tseng HR. Supramolecular nanosubstrate-mediated delivery system enables CRISPR-Cas9 knockin of hemoglobin beta gene for hemoglobinopathies. Sci Adv. 2020 Oct 23;6(43):eabb7107.

16.   Sun N, Lee YT, Zhang RY, Kao R, Teng PC, Yang Y, Yang P, Wang JJ, Smalley M, Chen PJ, Kim M, Chou SJ, Bao L, Wang J, Zhang X, Qi D, Palomique J, Nissen N, Han SB, Sadeghi S, Finn RS, Saab S, Busuttil RW, Markovic D, Elashoff D, Yu HH, Li H, Heaney AP, Posadas E, You S, Yang JD, Pei R, Agopian VG, Tseng HR, Zhu Y. Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring. Nat Commun. 2020 Sep 7;11(1):4489.

15. Chen CF, Chien CH, Yang YP, Chou SJ, Wang ML, Huo TI, Lin CC. Role of Dipeptidyl Peptidase 4 Inhibitors in Diabetic Patients with Coronavirus-19 Infection. J Chin Med Assoc. 2020 Apr 27.

14. Tu YF, Chien CS, Yarmishyn AA, Lin YY, Luo YH, Lin YT, Lai WY, Yang DM, Chou SJ, Yang YP, Wang ML, Chiou SH. A Review of SARS-CoV-2 and the Ongoing Clinical Trials. Int J Mol Sci. 2020 Apr 10;21(7). pii: E2657.

13. Yang TC, Yarmishyn AA, Yang YP, Lu PC, Chou SJ, Wang ML, Lin TC, Hwang DK, Chou YB, Chen SJ, Yu WK, Wang AG, Hsu CC, Chiou SH. Mitochondrial transport mediates survival of retinal ganglion cells in affected LHON patients. Hum Mol Genet. 2020 Apr 10. pii: ddaa063.

12. Shih-Jie Chou, * Peng Yang, * Qian Ban, Yi-Ping Yang, Mong-Lien Wang, Chian-Shiu Chien, Shih-Jen Chen, Na Sun, Yazhen Zhu, Hongtao Liu, Wenqiao Hui, Tai-Chi Lin, Fang Wang, Ryan Yue Zhang, Viet Q. Nguyen, Wenfei Liu, Mengxiang Chen, Steve J. Jonas, Paul S. Weiss, † Hsian-Rong Tseng, † and Shih-Hwa Chiou†. Dual Supramolecular Nanoparticle Vectors Enable CRISPR/Cas9-Mediated Knockin of Retinoschisin 1 Gene—A Potential Nonviral Therapeutic Solution for X-Linked Juvenile Retinoschisis. Adv. Sci. 2020 Apr 16, 1903432.

11. Tsai PH, Chien Y, Wang ML, Hsu CH, Laurent B, Chou SJ, Chang WC, Chien CS, Li HY, Lee HC, Huo TI, Hung JH, Chen CH, Chiou SH. Ash2l interacts with Oct4-stemness circuitry to promote super-enhancer-driven pluripotency network. Nucleic Acids Res. 2019 Nov 4;47(19):10115-10133.

10. Song HY, Chien CS, Yarmishyn AA, Chou SJ, Yang YP, Wang ML, Wang CY, Leu HB, Yu WC, Chang YL, Chiou SH. Generation of GLA-Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy. Cells. 2019 Apr 8;8(4). pii: E327.

9. Dong J, Zhang RY, Sun N, Smalley M, Wu Z, Zhou A, Chou SJ, Jan YJ, Yang P, Bao L, Qi D, Tang X, Tseng P, Hua Y, Xu D, Kao R, Meng M, Zheng X, Liu Y, Vagner T, Chai X, Zhou D, Li M, Chiou SH, Zheng G, Di Vizio D, Agopian VG, Posadas E, Jonas SJ, Ju SP, Weiss PS, Zhao M, Tseng HR, Zhu Y. Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer. ACS Appl Mater Interfaces. 2019 Apr 17;11(15):13973-13983.

8. Chou SJ, Ko YL, Yang YH, Yarmishyn AA, Wu YT, Chen CT, Lee HC, Wei YH, Chiou SH. Generation of two isogenic human induced pluripotent stem cell lines from a 15 year-old female patient with MERRF syndrome and A8344G mutation of mitochondrial DNA. Stem Cell Res. 2018 Jul;30:201-205.

7. Chien Y, Chou SJ, Chang YL, Leu HB, Yang YP, Tsai PH, Lai YH, Chen KH, Chang WC, Sung SH, Yu WC. Inhibition of Arachidonate 12/15-Lipoxygenase Improves α-Galactosidase Efficacy in iPSC-Derived Cardiomyocytes from Fabry Patients. Int J Mol Sci. 2018 May 16;19(5). pii: E1480.

6. Chou SJ, Yu WC, Chang YL, Chen WY, Chang WC, Chien Y, Yen JC, Liu YY, Chen SJ, Wang CY, Chen YH, Niu DM, Lin SJ, Chen JW, Chiou SH, Leu HB. Energy utilization of induced pluripotent stem cell-derived cardiomyocyte in Fabry disease. Int J Cardiol. 2017 Jan 5. pii: S0167-5273(17)30028-1.

5. Tseng WL*, Chou SJ*, Chiang HC, Wang ML, Chien CS, Chen KH, Leu HB, Wang CY, Chang YL, Liu YY, Jong YJ, Lin SZ, Chiou SH, Yu WC, Lin SJ. Imbalanced Production of Reactive Oxygen Species and Mitochondrial Antioxidant-SOD2 in Fabry Disease-specific Human Induced Pluripotent Stem Cell-differentiated Vascular Endothelial Cells. Cell Transplant. 2016 Dec 6.

4. Chien Y, Chien CS, Chiang HC, Huang WL, Chou SJ, Chang WC, Chang YL, Leu HB, Chen KH, Wang KL, Lai YH, Liu YY, Lu KH, Li HY, Sung YJ, Jong YJ, Chen YJ, Chen CH, Yu WC. Interleukin-18 deteriorates Fabry cardiomyopathy and contributes to the development of left ventricular hypertrophy in Fabry patients with GLA IVS4+919 G>A mutation. Oncotarget. 2016 Dec 27; 7(52):87161-87179.

3. Chou SJ, Tseng WL, Chen CT, Lai YF, Chien CS, Chang YL, Lee HC, Wei YH, Chiou SH. Impaired ROS Scavenging System in Human Induced Pluripotent Stem Cells Generated from Patients with MERRF Syndrome. Sci Rep. 2016 Mar 30;6:23661.

2. Tsai PH, Chien Y, Chuang JH, Chou SJ, Chien CH, Lai YH, Li HY, Ko YL, Chang YL, Wang CY, Liu YY, Lee HC, Yang CH, Tsai TF, Lee YY, Chiou SH. Dysregulation of Mitochondrial Functions and Osteogenic Differentiation in Cisd2-Deficient Murine Induced Pluripotent Stem Cells. Stem Cells Dev. 2015 Nov 1;24(21):2561-76.

1. Chiou SH, Jiang BH, Yu YL, Chou SJ, Tsai PH, Chang WC, Chen LK, Chen LH, Chien Y, Chiou GY. Poly(ADP-ribose) polymerase 1 regulates nuclear reprogramming and promotes iPSC generation without c-Myc. J Exp Med. 2013 Jan 14;210(1):85-98.